The prosocial motivation and behavior is important to build a harmonious society. This study do a survey to the students comes from eight colleges. The results indicate the students interpersonal trust are lower and the empathy level are average. In the helping situation, the students have higher level of helping willingness but lower level of helping time. The male and female have significant different in the prosocial motivation and behavior. At last, we suggest improving the situation safety and psychological safety, improving the empathy and interpersonal trust may increase the prosocial motivation and behavior.