抗日战争时期是毛泽东实事求是思想走向成熟与系统化的关键阶段。面对党内教条主义与经验主义的严重干扰，毛泽东立足于中国革命的具体实际，通过《实践论》《矛盾论》等哲学著作及延安整风运动，系统构建了以实事求是为核心的方法论体系。以抗日战争时期为历史场域，深入剖析毛泽东实事求是方法论的理论渊源、形成轨迹、核心内涵及实践路径，揭示其在马克思主义中国化进程中的关键作用。研究表明，实事求是方法论不仅为抗日战争的胜利提供了科学的思想武器，更为中国共产党思想路线的确立奠定了哲学基础，对新时代推进中国式现代化建设具有重要的方法论启示。

The Anti-Japanese War period was a critical stage in the maturation and systematization of Mao Zedong's pragmatic thought. Facing severe interference from dogmatism and empiricism within the Party, Mao Zedong, based on the specific realities of the China revolution, systematically constructed a methodological system centered on pragmatism through philosophical works such as "On Practice" and "On Contradiction," as well as the Yan' an Rectification Movement. Taking the Anti-Japanese War period as the historical context, this study conducts an in-depth analysis of the theoretical origins, formation trajectory, core connotations, and practical paths of Mao Zedong's pragmatic methodology, revealing its pivotal role in the Sinicization of Marxism. The research indicates that the pragmatic methodology not only provided a scientific ideological weapon for the victory of the Anti-Japanese War but also laid a philosophical foundation for the establishment of the Communist Party of China's ideological line, offering significant methodological insights for advancing Chinese-style modernization in the new era.