Filler is a kind of discourse marker which frequently appears Japanese spoken language. Its high frequency indeed to some extent creates barriers to the hearer in the understanding of the context, and someone even suggest that it should be eliminated. But on the other hand, the existence of fillers can indicate speaker’s psychological operations and information processing activities, making the conversation proceed more smoothly if properly grasped. Therefore its existence cannot be ignored. The study of fillers began in 1950, and has been growing since then. However, researches of filler aimed at Japanese language learners are scarcely seen, and there is no research on the usage of fillers based on differences of learners’ mother tongues. On the basis previous research, this study counts the occurrence number, occurrence rate and the situation of different vowel fillers of different leveled Japanese learners with the mother tongue of Chinese, English, Korean respectively. Then, the study uses SPSS multivariate analysis of variance to test whether there is a significant difference in the usage of vowel fillers when learners have different mother tongues.