In order to examine the level of physics learning interest of senior middle school Students and explore its differences in gender and grades, 871 senior middle students in Qufu are sampled as the subjects of the questionnaire survey in which the learning interest scale for senior middle school students in physics has been used. The results show: (1) The average score of senior middle school students is 19.082 on the whole learning interest scale. There is relatively high difference in the average scores among factors of the scale: the interest of Experiment Operation, Devotion, Expansion of Knowledge and Attention are higher, the interest of Cognition of Generalization and Cognition of Causality are lower. (2) There is significant gender difference in the physics learning interest of senior middle school Students. Boys’ learning interest is significantly higher than girls’. There exists certain difference in the physics learning interest of senior middle school students in grades. The interaction effect of grades ×gender is not significant.